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Two-dimensional DNA typinga parallel...
~
Uitterlinden, Andr歋 G.
Two-dimensional DNA typinga parallel approach to genome analysis /
紀錄類型:
書目-電子資源 : 單行本
正題名/作者:
Two-dimensional DNA typing/ Andr歋 G. Uitterlinden and Jan Vijg.
其他題名:
a parallel approach to genome analysis /
作者:
Uitterlinden, Andr歋 G.
其他作者:
Vijg, Jan.
出版者:
New York :E. Horwood,1994.
面頁冊數:
1 online resource (xii, 197 p.) :ill. (some col.)
標題:
DNA - Analysis. -
電子資源:
Click here for online access to this book (查閱全文) (EBSCO eBook)
ISBN:
0203168712 (electronic bk.)
ISBN:
9780203168714 (electronic bk.)
Two-dimensional DNA typinga parallel approach to genome analysis /
Uitterlinden, Andr歋 G.
Two-dimensional DNA typing
a parallel approach to genome analysis /[electronic resource] :Andr歋 G. Uitterlinden and Jan Vijg. - New York :E. Horwood,1994. - 1 online resource (xii, 197 p.) :ill. (some col.) - Ellis Horwood series in molecular biology. - Ellis Horwood series in molecular biology..
Includes bibliographical references (p. [164]-194) and index.
Two-dimensional DNA typing: background and technology -- Analysis of DNA: general aspects -- Measuring DNA sequence variation -- DNA sequencing -- Restriction enzyme digestion: RFLP analysis -- Analysis of basepairing -- Evaluation -- Measuring DNA sequence variation on a total genome scale -- Two-dimensional separation of DNA fragments -- Enzymatic digestion as second dimension separation criterion -- DGGE in the second dimension -- Two-dimensional DNA typing -- Summary and discussion: general characteristics of 2D DNA typing.
ISBN: 0203168712 (electronic bk.)Subjects--Topical Terms:
180336
DNA
--Analysis.Subjects--Index Terms:
DNAIndex Terms--Genre/Form:
172687
Electronic books.
LC Class. No.: QP624 / .T86 1994eb
Dewey Class. No.: 572.8636
National Agricultural Library Call No.: QP624.T86 / 1994
National Library of Medicine Call No.: 1994 D-149
Two-dimensional DNA typinga parallel approach to genome analysis /
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Two-dimensional DNA typing: background and technology -- Analysis of DNA: general aspects -- Measuring DNA sequence variation -- DNA sequencing -- Restriction enzyme digestion: RFLP analysis -- Analysis of basepairing -- Evaluation -- Measuring DNA sequence variation on a total genome scale -- Two-dimensional separation of DNA fragments -- Enzymatic digestion as second dimension separation criterion -- DGGE in the second dimension -- Two-dimensional DNA typing -- Summary and discussion: general characteristics of 2D DNA typing.
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Genome analysis: general considerations -- Mapping genomes -- Physical mapping -- Genetic mapping -- DNA markers -- Micro- and minisatellite sequences -- Other interspersed repetitive sequences -- Total genome analysis with DNA markers -- Combining genetic and physical mapping -- Strategies for identifying genetic traits -- Role of two-dimensional DNA typing in genome analysis -- General methodology -- Methods -- DNA isolation -- DNA digestion and sample preparation -- Electrophoretic separations -- Electrotransfer of separation patterns.
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Hybridization analysis -- Experimental considerations -- Denaturing gradient gel constellation -- Buffer dependency of separation patterns -- Reproducibility -- Pattern evaluation by image analysis -- Image capturing -- Spot detection -- Calibration -- Matching -- Database generation, searching and matching -- Applications -- Genome analysis of lower organisms -- Analysis of viral and bacterial genomes -- Analysis of lower eukaryotes -- Genetic linkage analysis -- Locus-specific melting behaviour of minisatellite alleles -- Anatomy of a 2D DNA typing gel -- Association studies.
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Genetic analysis of cancer -- Mutation analysis -- Future developments -- Positional 2D DNA typing: the concerted use of genetic and physical mapping -- Two-dimensional cDNA typing: analysing the expressed part of the genome -- Gene scanning: diagnosis of all possible mutations in disease genes.
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